A CT scan image of the spiral intestine of a Pacific spiny dogfish shark (Squalus suckleyi). The beginning of the intestine is on the left, and the end is on the right. Credit: Samantha Leigh/California State University Dominguez Hills
Contrary to what popular media portrays, we actually don’t know much about what sharks eat. Even less is known about how they digest their food, and the role they play in the larger ocean ecosystem.
For more than a century, researchers have relied on flat sketches of sharks’ digestive systems to discern how they function — and how what they eat and excrete impacts other species in the ocean. Now, researchers have produced a series of high-resolution, 3D scans of intestines from nearly three dozen shark species that will advance the understanding of how sharks eat and digest their food.
Three smooth dogfish sharks (Mustelus canis). Credit: Elizabeth Roberts/Wikimedia Commons
“It’s high time that some modern technology was used to look at these really amazing spiral intestines of sharks,” said lead author Samantha Leigh, assistant professor at California State University Dominguez Hills. “We developed a new method to digitally scan these tissues and now can look at the soft tissues in such great detail without having to slice into them.”
The research team from California State University Dominguez Hills, the University of Washington, and University of California, Irvine, published its findings July 21 in the journal Proceedings of the Royal Society B.
A CT scan image of a dogfish shark spiral intestine, shown from the top looking down. Credit: Samantha Leigh/California State University Dominguez Hills
The researchers primarily used a computerized tomography (CT) scanner at the UW’s Friday Harbor Laboratories to create 3D images of shark intestines, which came from specimens preserved at the Natural History Museum of Los Angeles. The machine works like a standard CT scanner used in hospitals: A series of X-ray images is taken from different angles, then combined using computer processing to create three-dimensional images. This allows researchers to see the complexities of a shark intestine without having to dissect or disturb it.
A live Pacific spiny dogfish shark (Squalus suckleyi). Credit: Samantha Leigh/California State University Dominguez Hills
“CT scanning is one of the only ways to understand the shape of shark intestines in three dimensions,” said co-author Adam Summers, a professor based at UW Friday Harbor Labs who has led a worldwide effort to scan the skeletons of fishes and other vertebrate animals. “Intestines are so complex — with so many overlapping layers, that dissection destroys the context and connectivity of the tissue. It would be like trying to understand what was reported in a newspaper by taking scissors to a rolled-up copy. The story just won’t hang together.”
A CT scan image of a smooth dogfish shark (Mustelus canis) spiral intestine, shown
from the top looking down. Credit: Samantha Leigh/California State University Dominguez Hills
From their scans, the researchers discovered several new aspects about how shark intestines function. It appears these spiral-shaped organs slow the movement of food and direct it downward through the gut, relying on gravity in addition to peristalsis, the rhythmic contraction of the gut’s smooth muscle. Its function resembles the one-way valve designed by Nikola Tesla more than a century ago that allows fluid to flow in one direction, without backflow or assistance from any moving parts.
This finding could shed new light on how sharks eat and process their food. Most sharks usually go days or even weeks between eating large meals, so they rely on being able to hold food in their system and absorb as many nutrients as possible, Leigh explained. The slowed movement of food through their gut caused by the spiral intestine probably allows sharks to retain their food longer, and they also use less energy processing that food.
This video shows the 3D image of a Pacific spiny dogfish (Squalus suckleyi) spiral intestine. Credit: Samantha Leigh/California State University Dominguez Hills
Because sharks are top predators in the ocean and also eat a lot of different things — invertebrates, fish, mammals and even seagrass — they naturally control the biodiversity of many species, the researchers said. Knowing how sharks process what they eat, and how they excrete waste, is important for understanding the larger ecosystem.
“The vast majority of shark species, and the majority of their physiology, are completely unknown. Every single natural history observation, internal visualization and anatomical investigation shows us things we could not have guessed at,” Summers said. “We need to look harder at sharks and, in particular, we need to look harder at parts other than the jaws, and the species that don’t interact with people.”
This video shows the soft tissue of a Pacific spiny dogfish (Squalus suckleyi) spiral intestine, rotated and viewed from different angles. Credit: Samantha Leigh/California State University Dominguez Hills
The authors plan to use a 3D printer to create models of several different shark intestines to test how materials move through the structures in real time. They also hope to collaborate with engineers to use shark intestines as inspiration for industrial applications such as wastewater treatment or filtering microplastics out of the water column.
Reference: 20 July 2021, Proceedings of the Royal Society B.
DOI: 10.1098/rspb.2021.1359
Other co-authors on the paper are Donovan German of University of California, Irvine, and Sarah Hoffmann of Applied Biological Services.
This research was funded by Friday Harbor Laboratories, the UC Irvine OCEANS Graduate Research Fellowship, the Newkirk Center Graduate Research Fellowship, the National Science Foundation Graduate Research Fellowship Program and UC Irvine.
